pet empty polycistronic destination vector 2e (Addgene inc)
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Addgene inc
pet empty polycistronic destination vector 2e
Pet Empty Polycistronic Destination Vector 2e, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet+empty+polycistronic+destination+vector+2e/pET+empty+polycistronic+destination+vector+(2E)+(Plasmid+%2329775)/pmc05562863-266-53-59
Average 86 stars, based on 1 article reviews
Pet Empty Polycistronic Destination Vector 2e, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet+empty+polycistronic+destination+vector+2e/pET+empty+polycistronic+destination+vector+(2E)+(Plasmid+%2329775)/pmc05562863-266-53-59
Average 86 stars, based on 1 article reviews
pet empty polycistronic destination vector 2e - by Bioz Stars,
2026-09
86/100 stars
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Clone Assay:Article Title: The inhibitory mechanism of Hal3 on the yeast Ppz1 phosphatase: A mutagenesis analysis Article Snippet: Alternatively, co-expression of Ppz1-Cter carrying selected mutations and Hal3 was carried out in E. coli using a polycistronic vector (T7 promoter), in a way that a 6xHis-tagged version of Ppz1-Cter is isolated by Ni-affinity chromatography and the co-expressed Hal3 protein is recovered on the basis of its capacity to interact with Ppz1-Cter. .. To this end, N-terminally 6xHis tagged Ppz1-Cter versions and Hal3 were cloned independently into pET His TEV LIC cloning vector (2B-T), a gift from Scott Gradia (Addgene plasmid # 29666), to provide the required ribosome binding sites, and then both ORFs were transferred to the multicloning site (cassettes 1 and 2) of the Cloning:Article Title: The inhibitory mechanism of Hal3 on the yeast Ppz1 phosphatase: A mutagenesis analysis Article Snippet: Alternatively, co-expression of Ppz1-Cter carrying selected mutations and Hal3 was carried out in E. coli using a polycistronic vector (T7 promoter), in a way that a 6xHis-tagged version of Ppz1-Cter is isolated by Ni-affinity chromatography and the co-expressed Hal3 protein is recovered on the basis of its capacity to interact with Ppz1-Cter. .. To this end, N-terminally 6xHis tagged Ppz1-Cter versions and Hal3 were cloned independently into pET His TEV LIC cloning vector (2B-T), a gift from Scott Gradia (Addgene plasmid # 29666), to provide the required ribosome binding sites, and then both ORFs were transferred to the multicloning site (cassettes 1 and 2) of the Plasmid Preparation:Article Title: The inhibitory mechanism of Hal3 on the yeast Ppz1 phosphatase: A mutagenesis analysis Article Snippet: Alternatively, co-expression of Ppz1-Cter carrying selected mutations and Hal3 was carried out in E. coli using a polycistronic vector (T7 promoter), in a way that a 6xHis-tagged version of Ppz1-Cter is isolated by Ni-affinity chromatography and the co-expressed Hal3 protein is recovered on the basis of its capacity to interact with Ppz1-Cter. .. To this end, N-terminally 6xHis tagged Ppz1-Cter versions and Hal3 were cloned independently into pET His TEV LIC cloning vector (2B-T), a gift from Scott Gradia (Addgene plasmid # 29666), to provide the required ribosome binding sites, and then both ORFs were transferred to the multicloning site (cassettes 1 and 2) of the Binding Assay:Article Title: The inhibitory mechanism of Hal3 on the yeast Ppz1 phosphatase: A mutagenesis analysis Article Snippet: Alternatively, co-expression of Ppz1-Cter carrying selected mutations and Hal3 was carried out in E. coli using a polycistronic vector (T7 promoter), in a way that a 6xHis-tagged version of Ppz1-Cter is isolated by Ni-affinity chromatography and the co-expressed Hal3 protein is recovered on the basis of its capacity to interact with Ppz1-Cter. .. To this end, N-terminally 6xHis tagged Ppz1-Cter versions and Hal3 were cloned independently into pET His TEV LIC cloning vector (2B-T), a gift from Scott Gradia (Addgene plasmid # 29666), to provide the required ribosome binding sites, and then both ORFs were transferred to the multicloning site (cassettes 1 and 2) of the |